mouse NCL shRNA silencing AAV

Name 
AAV-m-NCL-shRNA
Cat No 
shAAV-265514
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Ncl.

shAAV-265514
AAV-m-NCL-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Ncl (nucleolin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
B530004O11Rik; C23; D0Nds28; D1Nds28; Nucl
Description (Vector)
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5'-UUAGGG-3' repeats more tightly than the telomeric single-stranded DNA 5'-TTAGGG-3' repeats.
Gene ID
17975
Gene Name (Vector)
nucleolin
Gene Symbol
Ncl
ORF Size (aa)
2124
ORF Size (bp)
2124 bp
RefSeq ID
NM_010880
RefSeq Synonyms
NM_010880, BC005460,
Species
mouse
UniGene ID
Mm.474153

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.