mouse MSH2 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Msh2.
shAAV-265043
AAV-m-MSH2-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Msh2 (mutS homolog 2). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AI788990
Description (Vector)
Component of the post-replicative DNA mismatch repair system (MMR). Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Gene ID
17685
Gene Name (Vector)
mutS homolog 2
Gene Symbol
Msh2
ORF Size (aa)
2808
ORF Size (bp)
2808 bp
RefSeq ID
NM_008628
RefSeq Synonyms
NM_008628, BC050897, BC047117,
Species
mouse
UniGene ID
Mm.471165
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.