mouse LIME1 shRNA silencing AAV

Name 
AAV-m-LIME1-shRNA
Cat No 
shAAV-263456
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Lime1.

shAAV-263456
AAV-m-LIME1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Lime1 (Lck interacting transmembrane adaptor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2810038K19Rik; LIME
Description (Vector)
Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.
Gene ID
72699
Gene Name (Vector)
Lck interacting transmembrane adaptor 1
Gene Symbol
Lime1
ORF Size (aa)
810
ORF Size (bp)
810 bp
RefSeq ID
NM_023684
RefSeq Synonyms
NM_023684, BC023065,
Species
mouse
UniGene ID
Mm.440138

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.