mouse IRF3 shRNA silencing AAV

Name 
AAV-m-IRF3-shRNA
Cat No 
shAAV-262368
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Irf3.

shAAV-262368
AAV-m-IRF3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Irf3 (interferon regulatory factor 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
C920001K05Rik; interferon regulatory factor 3; IRF-3
Description (eCommerce)
Interferon regulatory factor 3 (IRF3) is a member of the IRF family of transcription factors that plays a critical role in immune cell function and development. IRF3 mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity.
Description (Vector)
Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
Gene ID
54131
Gene Name (eCommerce)
interferon regulatory factor 3
Gene Name (Vector)
interferon regulatory factor 3
Gene Symbol
Irf3
NCBI Taxonomy ID (eCommerce)
10090.0
ORF Size (aa)
1260
ORF Size (bp)
1260 bp
Protein Name (eCommerce)
Interferon regulatory factor 3
RefSeq ID
NM_016849
RefSeq Synonyms
NM_016849, BC050882,
Research Areas
Apoptosis,Signal Transduction
Research Areas (Faceted)
signal_transduction,cell_biology
Species
mouse
Target Sentence
Interferon regulatory factor 3 (IRF3) is a member of the IRF family of transcription factors that plays a critical role in immune cell function and development. IRF3 mediates interferon-stimulated response element (ISRE) promoter activation and functions as a molecular switch for antiviral activity.
UniGene ID
Mm.3960
UniProt ID (eCommerce)
P70671

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.