mouse NHEJ1 shRNA silencing AAV

Name 
AAV-m-NHEJ1-shRNA
Cat No 
shAAV-262061
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Nhej1.

shAAV-262061
AAV-m-NHEJ1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Nhej1 (nonhomologous end-joining factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1700029B21Rik; cernunnos; XLF
Description (Vector)
DNA repair protein involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. May serve as a bridge between XRCC4 and the other NHEJ factors located at DNA ends, or may participate in reconfiguration of the end bound NHEJ factors to allow XRCC4 access to the DNA termini. It may act in concert with XRCC6/XRCC5 (Ku) to stimulate XRCC4-mediated joining of blunt ends and several types of mismatched ends that are noncomplementary or partially complementary.
Gene ID
75570
Gene Name (Vector)
nonhomologous end-joining factor 1
Gene Symbol
Nhej1
ORF Size (aa)
888
ORF Size (bp)
888 bp
RefSeq ID
NM_029342
RefSeq Synonyms
NM_029342, BC132361, BC132359, BC107253, BC107252, BC006063,
Species
mouse
UniGene ID
Mm.439736

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.