mouse HS3ST3A1 shRNA silencing AAV

Name 
AAV-m-HS3ST3A1-shRNA
Cat No 
shAAV-261672
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Hs3st3a1.

shAAV-261672
AAV-m-HS3ST3A1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Hs3st3a1 (heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
3Ost3a; Hs3st3a
Description (Vector)
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Catalyzes the O-sulfation of glucosamine in IdoUA2S-GlcNS and also in IdoUA2S-GlcNH2. The substrate-specific O-sulfation generates an enzyme-modified heparan sulfate which acts as a binding receptor to Herpes simplex virus-1 (HSV-1) and permits its entry. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate.
Gene ID
15478
Gene Name (Vector)
heparan sulfate (glucosamine) 3-O-sulfotransferase 3A1
Gene Symbol
Hs3st3a1
ORF Size (aa)
1182
ORF Size (bp)
1182 bp
RefSeq ID
NM_178870
RefSeq Synonyms
NM_178870, BC094320,
Species
mouse
UniGene ID
Mm.340214

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.