mouse GM672 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Ctif.
shAAV-260306
AAV-m-GM672-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Ctif (CBP80/20-dependent translation initiation factor). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Gm672
Description (Vector)
Specifically required for the pioneer round of mRNA translation mediated by the cap-binding complex (CBC), that takes place during or right after mRNA export via the nuclear pore complex (NPC). Acts via its interaction with the NCBP1/CBP80 component of the CBC complex and recruits the 40S small subunit of the ribosome via eIF3. In contrast, it is not involved in steady state translation, that takes place when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. Also required for nonsense-mediated mRNA decay (NMD), the pioneer round of mRNA translation mediated by the cap-binding complex playing a central role in nonsense-mediated mRNA decay (NMD).
Gene ID
269037
Gene Name (Vector)
CBP80/20-dependent translation initiation factor
Gene Symbol
Ctif
ORF Size (aa)
1872
ORF Size (bp)
1872 bp
RefSeq ID
NM_201354
RefSeq Synonyms
NM_201354, BC058104,
Species
mouse
UniGene ID
Mm.36745
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.