mouse FLT1 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Flt1.
shAAV-259481
AAV-m-FLT1-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Flt1 (FMS-like tyrosine kinase 1). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
AI323757; Flt-1; sFlt1; VEGFR-1; VEGFR1
Description (Vector)
Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts (in vitro). Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Likewise, isoforms lacking a transmembrane domain, such as isoform 2, isoform 3 and isoform 4, may function as decoy receptors for VEGFA. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Isoform 1 phosphorylates PLCG1. Promotes phosphorylation of AKT1 at 'Ser-473'. Promotes phosphorylation of PTK2/FAK1; YES1 and CBL. Isoform 7 mediates phosphorylation of SRC at 'Tyr-418' and promotes tumor cell invasion.
Gene ID
14254
Gene Name (Vector)
FMS-like tyrosine kinase 1
Gene Symbol
Flt1
ORF Size (aa)
2067
ORF Size (bp)
2067 bp
RefSeq ID
NM_010228
RefSeq Synonyms
NM_010228, BC029674,
Species
mouse
UniGene ID
Mm.389712
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.