mouse EIF3M shRNA silencing AAV

Name
AAV-m-EIF3M-shRNA

Cat No
shAAV-258220

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Eif3m.

shAAV-258220

AAV-m-EIF3M-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Eif3m (eukaryotic translation initiation factor 3, subunit M). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Ga17; Pcid1; Tango7

Description (Vector)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. May favor virus entry in case of infection with herpes simplex virus 1 (HSV1) or herpes simplex virus 2 (HSV2).

Gene ID

98221

Gene Name (Vector)

eukaryotic translation initiation factor 3, subunit M

Gene Symbol

Eif3m

ORF Size (aa)

1125

ORF Size (bp)

1125 bp

RefSeq ID

NM_145380

RefSeq Synonyms

NM_145380, BC116789, BC116787, BC103795, BC089568, BC005598,

Species

mouse

UniGene ID

Mm.379278

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.