mouse EIF2AK2 shRNA silencing AAV

Name
AAV-m-EIF2AK2-shRNA

Cat No
shAAV-258182

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Eif2ak2.

shAAV-258182

AAV-m-EIF2AK2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Eif2ak2 (eukaryotic translation initiation factor 2-alpha kinase 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

2310047A08Rik; 4732414G15Rik; AI467567; AI747578; Pkr; Prkr; Tik

Description (Vector)

Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. In addition to serine/threonine-protein kinase activity, also has tyrosine-protein kinase activity: phosphorylates CDK1 upon DNA damage. CDK1 phosphorylation triggers CDK1 polyubiquitination and subsequent proteolysis, thus leading to G2 arrest.

Gene ID

19106

Gene Name (Vector)

eukaryotic translation initiation factor 2-alpha kinase 2

Gene Symbol

Eif2ak2

ORF Size (aa)

1548

ORF Size (bp)

1548 bp

RefSeq ID

NM_011163

RefSeq Synonyms

NM_011163, BC016422,

Species

mouse

UniGene ID

Mm.378990

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.