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mouse DNMT3B shRNA silencing AAV

Name 
AAV-m-DNMT3B-shRNA
Cat No 
shAAV-257357
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Dnmt3b.

shAAV-257357
AAV-m-DNMT3B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Dnmt3b (DNA methyltransferase 3B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
MmuIIIB
Description (Vector)
Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. May preferentially methylates nucleosomal DNA within the nucleosome core region. May function as transcriptional co-repressor by associating with CBX4 and independently of DNA methylation. Seems to be involved in gene silencing (By similarity). In association with DNMT1 and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Isoforms 4 and 5 are probably not functional due to the deletion of two conserved methyltransferase motifs. Function as transcriptional corepressor by associating with ZHX1.
Gene ID
13436
Gene Name (Vector)
DNA methyltransferase 3B
Gene Symbol
Dnmt3b
ORF Size (aa)
2583
ORF Size (bp)
2583 bp
RefSeq ID
NM_001003960
RefSeq Synonyms
NM_010068, NM_001271747, NM_001271746, NM_001271745, NM_001271744, NM_001122997, NM_001003963, NM_001003961, NM_001003960, BC105922, BC105677,
Species
mouse
UniGene ID
Mm.89772

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.