mouse DLAT shRNA silencing AAV

Name 
AAV-m-DLAT-shRNA
Cat No 
shAAV-257212
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Dlat.

shAAV-257212
AAV-m-DLAT-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Dlat (dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
6332404G05Rik; DLTA; PDC-E2
Description (Vector)
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).
Gene ID
235339
Gene Name (Vector)
dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex)
Gene Symbol
Dlat
ORF Size (aa)
1929
ORF Size (bp)
1929 bp
RefSeq ID
NM_145614
RefSeq Synonyms
NM_145614, BC069862, BC031495, BC026680,
Species
mouse
UniGene ID
Mm.285076

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.