mouse DHX58 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Dhx58.
shAAV-257170
AAV-m-DHX58-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Dhx58 (DEXH (Asp-Glu-X-His) box polypeptide 58). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
B430001I08Rik; D11Lgp2e; Lgp2; LPG2; RLR-3
Description (Vector)
Acts as a regulator of DDX58/RIG-I and IFIH1/MDA5 mediated antiviral signaling. Cannot initiate antiviral signaling as it lacks the CARD domain required for activating MAVS/IPS1-dependent signaling events. Can have both negative and positive regulatory functions related to DDX58/RIG-I and IFIH1/MDA5 signaling and this role in regulating signaling may be complex and could probably depend on characteristics of the infecting virus or target cells, or both. Its inhibitory action on DDX58/RIG-I signaling may involve the following mechanisms: competition with DDX58/RIG-I for binding to the viral RNA, binding to DDX58/RIG-I and inhibiting its dimerization and interaction with MAVS/IPS1, competing with IKBKE in its binding to MAVS/IPS1 thereby inhibiting activation of interferon regulatory factor 3 (IRF3). Its positive regulatory role may involve unwinding or stripping nucleoproteins of viral RNA thereby facilitating their recognition by DDX58/RIG-I and IFIH1/MDA5. Involved in the innate immune response to various RNA viruses and some DNA viruses such as poxviruses, and also to the bacterial pathogen Listeria monocytogenes. Can bind both ssRNA and dsRNA, with a higher affinity for dsRNA. Shows a preference to 5'-triphosphorylated RNA, although it can recognize RNA lacking a 5'-triphosphate.
Gene ID
80861
Gene Name (Vector)
DEXH (Asp-Glu-X-His) box polypeptide 58
Gene Symbol
Dhx58
ORF Size (aa)
2037
ORF Size (bp)
2037 bp
RefSeq ID
NM_030150
RefSeq Synonyms
NM_030150, BC029209,
Species
mouse
UniGene ID
Mm.271830
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.