mouse DGKZ shRNA silencing AAV

Name 
AAV-m-DGKZ-shRNA
Cat No 
shAAV-257124
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Dgkz.

shAAV-257124
AAV-m-DGKZ-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Dgkz (diacylglycerol kinase zeta). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
E130307B02Rik; ENSMUSG00000075035; F730209L11Rik; mDGK[z]
Description (Vector)
Displays a strong preference for 1,2-diacylglycerols over 1,3-diacylglycerols, but lacks substrate specificity among molecular species of long chain diacylglycerols. Isoform 2 but not isoform 1 regulates RASGRP1 activity.
Gene ID
104418
Gene Name (Vector)
diacylglycerol kinase zeta
Gene Symbol
Dgkz
ORF Size (aa)
2790
ORF Size (bp)
2790 bp
RefSeq ID
NM_138306
RefSeq Synonyms
NM_138306, NM_001166597, BC049228,
Species
mouse
UniGene ID
Mm.314923

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.