mouse CXCL12 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Cxcl12.
shAAV-256397
AAV-m-CXCL12-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Cxcl12 (chemokine (C-X-C motif) ligand 12). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Pbsf; Scyb12; Sdf1; Tlsf; Tpar1
Description (Vector)
Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1-alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3-67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T-cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.
Gene ID
20315
Gene Name (Vector)
chemokine (C-X-C motif) ligand 12
Gene Symbol
Cxcl12
ORF Size (aa)
270
RefSeq ID
NM_013655
RefSeq Synonyms
NM_021704, NM_013655, NM_001012477, BC006640,
Species
mouse
UniGene ID
Mm.303231
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.