mouse CTCFL shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Mouse Ctcfl.
shAAV-256292
AAV-m-CTCFL-shRNA
Ready-to-use AAV expressing shRNA for silencing of Mouse Ctcfl (CCCTC-binding factor (zinc finger protein)-like). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
Boris; OTTMUSG00000016680
Description (Vector)
Testis-specific DNA binding protein responsible for insulator function, nuclear architecture and transcriptional control, which probably acts by recruiting epigenetic chromatin modifiers. Plays a key role in gene imprinting in male germline, by participating in the establishment of differential methylation at the IGF2/H19 imprinted control region (ICR). Directly binds the unmethylated H19 ICR and recruits the PRMT7 methyltransferase, leading to methylate histone H4 'Arg-3' to form H4R3sme2. This probably leads to recruit de novo DNA methyltransferases at these sites (By similarity). Seems to act as tumor suppressor. In association with DNMT1 and DNMT3B, involved in activation of BAG1 gene expression by binding to its promoter. Required for dimethylation of H3 lysine 4 (H3K4me2) of MYC and BRCA1 promoters.
Gene ID
664799
Gene Name (Vector)
CCCTC-binding factor (zinc finger protein)-like
Gene Symbol
Ctcfl
ORF Size (aa)
1908
ORF Size (bp)
1908 bp
RefSeq ID
NM_001081387
RefSeq Synonyms
NM_001081387, BC152845, BC151026,
Species
mouse
UniGene ID
Mm.389396
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.