mouse CAST shRNA silencing AAV

Name 
AAV-m-CAST-shRNA
Cat No 
shAAV-254577
Availability
Immediate
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Cast.

shAAV-254577
AAV-m-CAST-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Cast (calpastatin). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Description (Vector)
Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
Gene ID
12380
Gene Name (Vector)
calpastatin
Gene Symbol
Cast
ORF Size (aa)
2025
ORF Size (bp)
2025 bp
RefSeq ID
NM_009817
RefSeq Synonyms
NM_009817, NM_001301181, NM_001301160, NM_001301158, NM_001301157, NM_001301156, NM_001301155, NM_001301153, BC010603,
Species
mouse
UniGene ID
Mm.441995

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.