mouse BLM shRNA silencing AAV

Name
AAV-m-BLM-shRNA

Cat No
shAAV-253995

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Blm.

shAAV-253995

AAV-m-BLM-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Blm (Bloom syndrome, RecQ helicase-like). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

Bloom syndrome homolog; Bloom syndrome protein homolog; Bloom syndrome, RecQ helicase-like; mBLM; recQ helicase homolog

Description (eCommerce)

Bloom syndrome protein (BLM) is a RecQ DNA-helicase that plays a role in DNA replication and repair. Mutations and inactivation of BLM result in human syndromes characterized by dwarfism, infertility, erythema, learning disabilities, immune deficiency and predisposition to cancer. BLM has been shown to associate with the BRCA1-associated genome surveillance complex (BASC) which contains BRCA1, MSH2, MSH6, MLH1, ATM, PMS2 and the RAD50-MRE11-NBS1 protein complex.

Description (Vector)

Participates in DNA replication and repair. Exhibits a magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction.

Gene ID

12144

Gene Name (eCommerce)

Bloom syndrome, RecQ like helicase

Gene Name (Vector)

Bloom syndrome, RecQ helicase-like

Gene Symbol

Blm

NCBI Taxonomy ID (eCommerce)

10090.0

ORF Size (aa)

4251

ORF Size (bp)

4251 bp

Protein Name (eCommerce)

Bloom syndrome protein homolog

RefSeq ID

NM_007550

RefSeq Synonyms

NM_007550, NM_001042527, BC157095,

Research Areas

Breast Cancer,Cancer,Cell Cycle,DNA Damage/Repair,DNA Replication,Fanconi anemia,Helicase Activity,Homologous Recombination,Immunology,Prostate Cancer,Replication,Telomeric Maintenance,Tumor Suppressors/Oncoproteins

Research Areas (Faceted)

cancer,cell_biology,cell_cycle,genetics,transcription_translation,immunology

Species

mouse

Target Sentence

Bloom syndrome protein (BLM) is a RecQ DNA-helicase that plays a role in DNA replication and repair. Mutations and inactivation of BLM result in human syndromes characterized by dwarfism, infertility, erythema, learning disabilities, immune deficiency and predisposition to cancer. BLM has been shown to associate with the BRCA1-associated genome surveillance complex (BASC) which contains BRCA1, MSH2, MSH6, MLH1, ATM, PMS2 and the RAD50-MRE11-NBS1 protein complex.

UniGene ID

Mm.12932

UniProt ID (eCommerce)

O88700

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.