mouse ATP5J shRNA silencing AAV

Name
AAV-m-ATP5J-shRNA

Cat No
shAAV-253307

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Mouse Atp5j.

shAAV-253307

AAV-m-ATP5J-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Atp5j (ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

CF6

Description (Vector)

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements. Also involved in the restoration of oligomycin-sensitive ATPase activity to depleted F1-F0 complexes.

Gene ID

11957

Gene Name (Vector)

ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F

Gene Symbol

Atp5j

ORF Size (aa)

327

RefSeq ID

NM_016755

RefSeq Synonyms

NM_016755, NM_001302216, NM_001302215, NM_001302214, NM_001302213, BC010766,

Species

mouse

UniGene ID

Mm.353

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.