mouse ATP5B shRNA silencing AAV

Name
AAV-m-ATP5B-shRNA

Cat No
shAAV-253293

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Mouse Atp5b.

shAAV-253293

AAV-m-ATP5B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Atp5b (ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Description (Vector)

Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits.

Gene ID

11947

Gene Name (Vector)

ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit

Gene Symbol

Atp5b

ORF Size (aa)

1590

ORF Size (bp)

1590 bp

RefSeq ID

NM_016774

RefSeq Synonyms

NM_016774, BC046616,

Species

mouse

UniGene ID

Mm.238973

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.