mouse ARFGAP1 shRNA silencing AAV

Name 
AAV-m-ARFGAP1-shRNA
Cat No 
shAAV-252911
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Arfgap1.

shAAV-252911
AAV-m-ARFGAP1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Arfgap1 (ADP-ribosylation factor GTPase activating protein 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AI115377; Arf1gap
Description (Vector)
GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).
Gene ID
228998
Gene Name (Vector)
ADP-ribosylation factor GTPase activating protein 1
Gene Symbol
Arfgap1
ORF Size (aa)
1179
ORF Size (bp)
1179 bp
RefSeq ID
NM_145760
RefSeq Synonyms
NM_145760, NM_001177710, NM_001177709, NM_001177708, NM_001177707, NM_001177706, BC059817, BC052922,
Species
mouse
UniGene ID
Mm.33765

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.