mouse APLF shRNA silencing AAV

Name 
AAV-m-APLF-shRNA
Cat No 
shAAV-252821
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse Aplf.

shAAV-252821
AAV-m-APLF-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse Aplf (aprataxin and PNKP like factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
2010301N04Rik; AI452191
Description (Vector)
Nuclease involved in single-strand and double-strand DNA break repair. Recruited to sites of DNA damage through interaction with poly(ADP-ribose), a polymeric post-translational modification synthesized transiently at sites of chromosomal damage to accelerate DNA strand break repair reactions. Displays apurinic-apyrimidinic (AP) endonuclease and 3'-5' exonuclease activities in vitro. Also able to introduce nicks at hydroxyuracil and other types of pyrimidine base damage.
Gene ID
72103
Gene Name (Vector)
aprataxin and PNKP like factor
Gene Symbol
Aplf
ORF Size (aa)
1176
ORF Size (bp)
1176 bp
RefSeq ID
NM_024251
RefSeq Synonyms
NM_024251, NM_001170489, BC002179,
Species
mouse
UniGene ID
Mm.31770

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.