mouse 1110034B05RIK shRNA silencing AAV

Name 
AAV-m-1110034B05RIK-shRNA
Cat No 
shAAV-250146
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Mouse TYW5.

shAAV-250146
AAV-m-1110034B05RIK-shRNA

Ready-to-use AAV expressing shRNA for silencing of Mouse TYW5 (tRNA-yW synthesizing protein 5). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
1110034B05Rik
Description (Vector)
tRNA hydroxylase that acts as a component of the wybutosine biosynthesis pathway. Wybutosine is a hyper modified guanosine with a tricyclic base found at the 3'-position adjacent to the anticodon of eukaryotic phenylalanine tRNA. Catalyzes the hydroxylation of 7-(a-amino-a-carboxypropyl)wyosine (yW-72) into undermodified hydroxywybutosine (OHyW*). OHyW* being further transformed into hydroxywybutosine (OHyW) by LCMT2/TYW4. OHyW is a derivative of wybutosine found in higher eukaryotes.
Gene ID
68736
Gene Name (Vector)
tRNA-yW synthesizing protein 5
Gene Symbol
TYW5
ORF Size (aa)
948
RefSeq ID
NM_001037742
RefSeq Synonyms
NM_001302964, NM_001302963, NM_001302962, NM_001114102, NM_001037742, BC132289,
Species
mouse
UniGene ID
Mm.288490

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.