human WDFY3 shRNA silencing AAV

Name 
AAV-h-WDFY3-shRNA
Cat No 
shAAV-234238
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human WDFY3.

shAAV-234238
AAV-h-WDFY3-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human WDFY3 (WD repeat and FYVE domain containing 3). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
ALFY; autophagy-linked FYVE protein; BCHS; MCPH18; WD repeat and FYVE domain-containing protein 3; ZFYVE25
Description (eCommerce)
WDFY3 (WD repeat and FYVE domain –containing protein 3) was identified in a screen for phosphatidylinositol -3-phosphate [PtdIns(3)P]- binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3)-binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery.
Description (Vector)
WDFY3 encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy.
Gene ID
23001
Gene Name (eCommerce)
WD repeat and FYVE domain containing 3
Gene Name (Vector)
WD repeat and FYVE domain containing 3
Gene Symbol
WDFY3
HGNC ID
HGNC:20751
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1315
Protein Name (eCommerce)
WD repeat and FYVE domain-containing protein 3
RefSeq ID
NM_014991
RefSeq Synonyms
NM_014991, BC015214,
Research Areas
Autophagy,Zinc-finger
Research Areas (Faceted)
cell_biology
Species
human
Target Sentence
WDFY3 (WD repeat and FYVE domain –containing protein 3) was identified in a screen for phosphatidylinositol -3-phosphate [PtdIns(3)P]- binding proteins. The FYVE domain is a zinc binding domain found in many PtdIns(3)-binding proteins and is proposed to target proteins to membrane lipids. WDFY3 has been shown localized to the nuclear membrane and within autophagic membranes and it is proposed to function as a link between protein aggregates and the autophagic machinery.
UniProt ID (eCommerce)
Q8IZQ1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.