human ACAT1 shRNA silencing AAV

Name
AAV-h-ACAT1-shRNA

Cat No
shAAV-233298

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human ACAT1.

shAAV-233298

AAV-h-ACAT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ACAT1 (acetyl-CoA acetyltransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

ACAT; MAT; T2; THIL

Description (Vector)

ACAT1 encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in this gene are associated with 3-ketothiolase deficiency, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Gene ID

Gene Name (Vector)

acetyl-CoA acetyltransferase 1

Gene Symbol

ACAT1

HGNC ID

HGNC:93

ORF Size (aa)

489

ORF Size (bp)

489 bp

RefSeq ID

NM_000019

RefSeq Synonyms

NM_000019, BC010942,

Species

human

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.