human ASCL1 shRNA silencing AAV

Name 
AAV-h-ASCL1-shRNA
Cat No 
shAAV-229295
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ASCL1.

shAAV-229295
AAV-h-ASCL1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ASCL1 (achaete-scute family bHLH transcription factor 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
achaete scute protein; achaete-scute complex homolog 1; achaete-scute complex-like 1; achaete-scute homolog 1; ASH1; ASH-1; bHLHa46; class A basic helix-loop-helix protein 46; HASH1; MASH1
Description (eCommerce)
ASCL1/MASH1 is a member of the basic helix-loop-helix (BHLH) family of transcription factors. Dimerization with other BHLH proteins is required for efficient DNA binding. ASCL1 plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. [taken from NCBI Entrez Gene (Gene ID: 429)].
Description (Vector)
ASCL1 encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases.
Gene ID
429
Gene Name (eCommerce)
achaete-scute family bHLH transcription factor 1
Gene Name (Vector)
achaete-scute family bHLH transcription factor 1
Gene Symbol
ASCL1
HGNC ID
HGNC:738
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
711
ORF Size (bp)
711 bp
Protein Name (eCommerce)
Achaete-scute homolog 1
RefSeq ID
NM_004316
RefSeq Synonyms
NM_004316, BC031299, BC004425, BC003134, BC002341,
Research Areas
Apoptosis,Neurobiology,Neurodevelopment,Neurogenesis,Notch Signaling,Signal Transduction,Transcription Factor/Regulator
Research Areas (Faceted)
cell_biology,developmental_biology,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
ASCL1/MASH1 is a member of the basic helix-loop-helix (BHLH) family of transcription factors. Dimerization with other BHLH proteins is required for efficient DNA binding. ASCL1 plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. [taken from NCBI Entrez Gene (Gene ID: 429)].
UniGene ID
Hs.704281
UniProt ID (eCommerce)
P50553

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.