human ZFYVE9 shRNA silencing AAV

Name
AAV-h-ZFYVE9-shRNA

Cat No
shAAV-228244

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human ZFYVE9.

shAAV-228244

AAV-h-ZFYVE9-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ZFYVE9 (zinc finger, FYVE domain containing 9). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

hSARA; MAD, mothers against decapentaplegic homolog interacting protein, receptor activation anchor; MADH-interacting protein; MADHIP; Mothers against decapentaplegic homolog-interacting protein; novel serine protease; NSP; PPP1R173; protein phosphatase 1, regulatory subunit 173; Receptor activation anchor; SARA; smad anchor for receptor activation; SMADIP; zinc finger FYVE domain-containing protein 9; zinc finger, FYVE domain containing 9

Description (eCommerce)

Smad anchor for receptor activation (SARA) is an early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. SARA plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. SARA is possibly associated with TGF-beta receptor internalization [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O95405].

Description (Vector)

ZFYVE9 encodes a double zinc finger (FYVE domain) protein that interacts directly with SMAD2 and SMAD3, and is involved in Alzheimer's disease. SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. The FYVE domain has been identified in a number of unrelated signaling molecules. This protein functions to recruit SMAD2 to the transforming growth factor-beta receptor. The FYVE domain is required to maintain the normal localization of this protein but is not involved in mediating interaction with SMADs. The C-terminal domain of this protein interacts with the TGFB receptor. This protein is a component of the TGFB pathway that brings the SMAD substrate to the receptor. Three alternatively spliced transcripts encoding distinct isoforms have been found for this gene.

Gene ID

9372

Gene Name (eCommerce)

zinc finger FYVE-type containing 9

Gene Name (Vector)

zinc finger, FYVE domain containing 9

Gene Symbol

ZFYVE9

HGNC ID

HGNC:6775

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

4278

ORF Size (bp)

4278 bp

Protein Name (eCommerce)

Zinc finger FYVE domain-containing protein 9

RefSeq ID

NM_004799

RefSeq Synonyms

NM_007324, NM_007323, NM_004799, BC032680,

Research Areas

Signal Transduction,TGF-beta Signaling,Transport,Zinc-finger

Research Areas (Faceted)

signal_transduction,signal_transduction,immunology,cell_biology

Species

human

Target Sentence

UniGene ID

Hs.532345

UniProt ID (eCommerce)

O95405

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.