human ZEB2 shRNA silencing AAV

Name 
AAV-h-ZEB2-shRNA
Cat No 
shAAV-228178
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ZEB2.

shAAV-228178
AAV-h-ZEB2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ZEB2 (zinc finger E-box binding homeobox 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
HSPC082; SIP1; SIP-1; SMAD interacting protein 1; Smad-interacting protein 1; SMADIP1; ZFHX1B; zinc finger E-box-binding homeobox 2; zinc finger homeobox 1b; Zinc finger homeobox protein 1b
Description (eCommerce)
Smad interacting protein 1 (SIP1) is a transcriptional inhibitor that binds to the DNA sequence, 5'-CACCT-3', in different promoters. SIP1 has been shown to repress the transcription of E-cadherin. Defects in the gene which encodes for SIP1, ZEB2, are the cause of Hirschsprung disease-mental retardation syndrome (Hirschsprung disease) also known as Mowat-Wilson syndrome (MWS). Hirschsprung disease is a rare autosomal dominant complex developmental disorder [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60315].
Description (Vector)
ZEB2 is a member of the Zfh1 family of 2-handed zinc finger/homeodomain proteins. It is located in the nucleus and functions as a DNA-binding transcriptional repressor that interacts with activated SMADs. Mutations in this gene are associated with Hirschsprung disease/Mowat-Wilson syndrome. Alternatively spliced transcript variants have been found for this gene.
Gene ID
9839
Gene Name (eCommerce)
zinc finger E-box binding homeobox 2
Gene Name (Vector)
zinc finger E-box binding homeobox 2
Gene Symbol
ZEB2
HGNC ID
HGNC:14881
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
3645
ORF Size (bp)
3645 bp
Protein Name (eCommerce)
Zinc finger E-box-binding homeobox 2
RefSeq ID
NM_014795
RefSeq Synonyms
NM_014795, NM_001171653, BC127102, BC127101,
Research Areas
Cancer,Epilepsy,JNK Signaling,MicroRNAs in Cancer,Neurobiology,Neurodevelopment,Signal Transduction,TGF-beta Signaling,Transcription Factor/Regulator,Wnt Signaling,Zinc-finger
Research Areas (Faceted)
cancer,genetics,signal_transduction,neurobiology,neurobiology,developmental_biology,signal_transduction,immunology,genetics,transcription_translation,developmental_biology,cell_biology
Species
human
Target Sentence
Smad interacting protein 1 (SIP1) is a transcriptional inhibitor that binds to the DNA sequence, 5'-CACCT-3', in different promoters. SIP1 has been shown to repress the transcription of E-cadherin. Defects in the gene which encodes for SIP1, ZEB2, are the cause of Hirschsprung disease-mental retardation syndrome (Hirschsprung disease) also known as Mowat-Wilson syndrome (MWS). Hirschsprung disease is a rare autosomal dominant complex developmental disorder [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/O60315].
UniGene ID
Hs.34871
UniProt ID (eCommerce)
O60315

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.