human WDR35 shRNA silencing AAV

Name 
AAV-h-WDR35-shRNA
Cat No 
shAAV-227664
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human WDR35.

shAAV-227664
AAV-h-WDR35-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human WDR35 (WD repeat domain 35). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CED2; IFT121; IFTA1; SRTD7
Description (Vector)
WDR35 encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. Two patients with Sensenbrenner syndrome / cranioectodermal dysplasia (CED) were identified with mutations in this gene, consistent with a possible ciliary function.
Gene ID
57539
Gene Name (Vector)
WD repeat domain 35
Gene Symbol
WDR35
HGNC ID
HGNC:29250
ORF Size (aa)
3513
ORF Size (bp)
3513 bp
RefSeq ID
NM_020779
RefSeq Synonyms
NM_020779, NM_001006657, BC036659,
Species
human
UniGene ID
Hs.205427

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.