human VEGFA shRNA silencing AAV

Name
AAV-h-VEGFA-shRNA

Cat No
shAAV-227457

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human VEGFA.

shAAV-227457

AAV-h-VEGFA-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human VEGFA (vascular endothelial growth factor A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

MVCD1; VEGF; VPF

Description (Vector)

VEGFA is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms.

Gene ID

7422

Gene Name (Vector)

vascular endothelial growth factor A

Gene Symbol

VEGFA

HGNC ID

HGNC:12680

ORF Size (aa)

1188

RefSeq ID

NM_003376

RefSeq Synonyms

NM_003376, NM_001317010, NM_001287044, NM_001204385, NM_001204384, NM_001171630, NM_001171629, NM_001171628, NM_001171627, NM_001171626, NM_001171625, NM_001171624, NM_001171623, NM_001171622, NM_001033756, NM_001025370, NM_001025369, NM_001025368, NM_001025367, NM_001025366, BC172307, BC065522,

Species

human

UniGene ID

Hs.73793

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.