human UNC13B shRNA silencing AAV

Name
AAV-h-UNC13B-shRNA

Cat No
shAAV-227183

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human UNC13B.

shAAV-227183

AAV-h-UNC13B-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human UNC13B (unc-13 homolog B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

MUNC13; UNC13; Unc13h2

Description (Vector)

UNC13B is expressed in the kidney cortical epithelial cells and is upregulated by hyperglycemia. The encoded protein shares a high level of similarity to the rat homolog, and contains 3 C2 domains and a diacylglycerol-binding C1 domain. Hyperglycemia increases the levels of diacylglycerol, which has been shown to induce apoptosis in cells transfected with this gene and thus contribute to the renal cell complications of hyperglycemia. Studies in other species also indicate a role for this protein in the priming step of synaptic vesicle exocytosis.

Gene ID

10497

Gene Name (Vector)

unc-13 homolog B

Gene Symbol

UNC13B

HGNC ID

HGNC:12566

ORF Size (aa)

3594

ORF Size (bp)

3594 bp

RefSeq ID

NM_006377

RefSeq Synonyms

NM_006377, NM_001330653, BC111800, BC111781,

Species

human

UniGene ID

Hs.493791

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.