human UBE3B shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human UBE3B.
shAAV-227049
AAV-h-UBE3B-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human UBE3B (ubiquitin protein ligase E3B). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
BPIDS; KOS
Description (Vector)
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: E1 ubiquitin-activating enzymes, E2 ubiquitin-conjugating enzymes, and E3 ubiquitin-protein ligases. This gene encodes a member of the E3 ubiquitin-conjugating enzyme family which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme and transfers the ubiquitin to the targeted substrates. A HECT (homology to E6-AP C-terminus) domain in the C-terminus of the longer isoform of this protein is the catalytic site of ubiquitin transfer and forms a complex with E2 conjugases. Shorter isoforms of this protein which lack the C-terminal HECT domain are therefore unlikely to bind E2 enzymes. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
Gene ID
89910
Gene Name (Vector)
ubiquitin protein ligase E3B
Gene Symbol
UBE3B
HGNC ID
HGNC:13478
ORF Size (aa)
3207
ORF Size (bp)
3207 bp
RefSeq ID
NM_130466
RefSeq Synonyms
NM_183415, NM_130466, NM_001270451, NM_001270450, NM_001270449, BC150637, BC141880, BC051266,
Species
human
UniGene ID
Hs.374067
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.