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human UBE2W shRNA silencing AAV

Name 
AAV-h-UBE2W-shRNA
Cat No 
shAAV-227045
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human UBE2W.

shAAV-227045
AAV-h-UBE2W-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human UBE2W (ubiquitin conjugating enzyme E2 W (putative)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
UBC-16; UBC16
Description (Vector)
UBE2W encodes a nuclear-localized ubiquitin-conjugating enzyme (E2) that, along with ubiquitin-activating (E1) and ligating (E3) enzymes, coordinates the addition of a ubiquitin moiety to existing proteins. The encoded protein promotes the ubiquitination of Fanconi anemia complementation group proteins and may be important in the repair of DNA damage. There is a pseudogene for this gene on chromosome 1. Alternative splicing results in multiple transcript variants.
Gene ID
55284
Gene Name (Vector)
ubiquitin conjugating enzyme E2 W (putative)
Gene Symbol
UBE2W
HGNC ID
HGNC:25616
ORF Size (aa)
480
RefSeq ID
NM_018299
RefSeq Synonyms
NM_018299, NM_001271015, NM_001001481, BC016326, BC010900,
Species
human
UniGene ID
Hs.597486

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.