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human TSEN54 shRNA silencing AAV

Name 
AAV-h-TSEN54-shRNA
Cat No 
shAAV-226594
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human TSEN54.

shAAV-226594
AAV-h-TSEN54-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TSEN54 (tRNA splicing endonuclease subunit 54). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
PCH2A; PCH4; PCH5; sen54; SEN54L
Description (Vector)
TSEN54 encodes a subunit of the tRNA splicing endonuclease complex, which catalyzes the removal of introns from precursor tRNAs. The complex is also implicated in pre-mRNA 3-prime end processing. Mutations in this gene result in pontocerebellar hypoplasia type 2.
Gene ID
283989
Gene Name (Vector)
tRNA splicing endonuclease subunit 54
Gene Symbol
TSEN54
HGNC ID
HGNC:27561
ORF Size (aa)
1581
ORF Size (bp)
1581 bp
RefSeq ID
NM_207346
RefSeq Synonyms
NM_207346, BC047793,
Species
human
UniGene ID
Hs.655875

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.