human TSC2 shRNA silencing AAV

Name 
AAV-h-TSC2-shRNA
Cat No 
shAAV-226578
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human TSC2.

shAAV-226578
AAV-h-TSC2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TSC2 (tuberous sclerosis 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
LAM; PPP1R160; protein phosphatase 1, regulatory subunit 160; TSC4; tuberin; tuberous sclerosis 2 protein
Description (eCommerce)
Tuberous sclerosis 2 protein (TSC2) forms a heterodimer with TSC1 to integrate cellular growth and stress signals. Defects in the TSC1 and TSC2 gene are responsible for the genetic disorder tuberous sclerosis. The TSC1/TSC2 complex functions in the mTOR pathway by negatively regulating mTOR activity via the small GTP-binding protein Rheb. TSC2 also been shown to interact with FOXO1 transcription factor.
Description (Vector)
Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.
Gene ID
7249
Gene Name (eCommerce)
TSC complex subunit 2
Gene Name (Vector)
tuberous sclerosis 2
Gene Symbol
TSC2
HGNC ID
HGNC:12363
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
5355
ORF Size (bp)
5355 bp
Protein Name (eCommerce)
Tuberin
RefSeq ID
NM_000548
RefSeq Synonyms
NM_001318832, NM_001318831, NM_001318829, NM_001318827, NM_001114382, NM_001077183, NM_000548, BC150300,
Research Areas
B-cell Receptor Signaling,BDNF Signaling,Breast Cancer,Cancer,Cardiac Development,Cardiology,Cardiovascular,Cell Cycle,Cytoskeleton,EGFR Signaling,EGFR Signaling in Cancer,Fc Receptor Signaling,FGF Signaling,Glioma,Golgi Apparatus,Hypoxia,IGF-1 Signaling,Immunology,Innate Immunity,Insulin Signaling,Kinase/Phosphatase,LKB1 Signaling,MAPK Signaling,mTOR Signaling,Neurobiology,Neurodevelopment,NGF Signaling,p53 Signaling,PDGF Signaling,PI3K/Akt Signaling,PI3K-AKT Signaling in Cancer,Prostate Cancer,SCF-KIT Signaling,Signal Transduction,Tumor Suppressors/Oncoproteins,Wnt Signaling
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,immunology,neurobiology,signal_transduction,stem_cell_biology
Species
human
Target Sentence
Tuberous sclerosis 2 protein (TSC2) forms a heterodimer with TSC1 to integrate cellular growth and stress signals. Defects in the TSC1 and TSC2 gene are responsible for the genetic disorder tuberous sclerosis. The TSC1/TSC2 complex functions in the mTOR pathway by negatively regulating mTOR activity via the small GTP-binding protein Rheb. TSC2 also been shown to interact with FOXO1 transcription factor.
UniGene ID
Hs.90303
UniProt ID (eCommerce)
P49815

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.