human TSC1 shRNA silencing AAV

Name
AAV-h-TSC1-shRNA

Cat No
shAAV-226577

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human TSC1.

shAAV-226577

AAV-h-TSC1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human TSC1 (tuberous sclerosis 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

90

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

hamartin; LAM; truncated hemartin; TSC; tuberous sclerosis 1 protein

Description (eCommerce)

Tuberous sclerosis 1 protein (TSC1) forms a heterodimer with TSC2 to integrate cellular growth and stress signals. Defects in the TSC1 and TSC2 gene are responsible for the genetic disorder tuberous sclerosis. The TSC1/TSC2 complex functions in the mTOR pathway by negatively regulating mTOR activity via the small GTP-binding protein Rheb.

Description (Vector)

TSC1 encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants.

Gene ID

7248

Gene Name (eCommerce)

TSC complex subunit 1

Gene Name (Vector)

tuberous sclerosis 1

Gene Symbol

TSC1

HGNC ID

HGNC:12362

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

3495

ORF Size (bp)

3495 bp

Protein Name (eCommerce)

Hamartin

RefSeq ID

NM_000368

RefSeq Synonyms

NM_001162427, NM_001162426, NM_000368, BC167824,

Research Areas

Breast Cancer,Cancer,Cardiac Development,Cardiology,Cardiovascular,Cell Cycle,Epilepsy,Glioma,IGF-1 Signaling,Insulin Signaling,LKB1 Signaling,mTOR Signaling,Neurobiology,Neurodevelopment,Nuclear Import/Export,PI3K/Akt Signaling,Prostate Cancer,Signal Transduction,Translational Control,Transport,Tumor Suppressors/Oncoproteins,Ubiquitination,Wnt Signaling

Research Areas (Faceted)

cancer,cardiovascular,cell_biology,cell_cycle,developmental_biology,genetics,neurobiology,signal_transduction,transcription_translation

Species

human

Target Sentence

Tuberous sclerosis 1 protein (TSC1) forms a heterodimer with TSC2 to integrate cellular growth and stress signals. Defects in the TSC1 and TSC2 gene are responsible for the genetic disorder tuberous sclerosis. The TSC1/TSC2 complex functions in the mTOR pathway by negatively regulating mTOR activity via the small GTP-binding protein Rheb.

UniGene ID

Hs.370854

UniProt ID (eCommerce)

Q92574

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.