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human SUFU shRNA silencing AAV

Name 
AAV-h-SUFU-shRNA
Cat No 
shAAV-224700
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human SUFU.

shAAV-224700
AAV-h-SUFU-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SUFU (SUFU negative regulator of hedgehog signaling). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
JBTS32; PRO1280; SUFUH; SUFUXL; suppressor of fused homolog
Description (eCommerce)
Suppressor of fused (SuFu) is a negative regulator of the hedgehog and beta-catenin signaling pathway. It down-regulates GLI1-mediated transactivation of target genes and is part of a corepressor complex that acts on DNA-bound GLI1. SuFu may also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Additionally, SuFu sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UMX1].
Description (Vector)
The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.
Gene ID
51684
Gene Name (eCommerce)
SUFU negative regulator of hedgehog signaling
Gene Name (Vector)
SUFU negative regulator of hedgehog signaling
Gene Symbol
SUFU
HGNC ID
HGNC:16466
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1455
ORF Size (bp)
1455 bp
Protein Name (eCommerce)
Suppressor of fused homolog
RefSeq ID
NM_016169
RefSeq Synonyms
NM_016169, NM_001178133, BC013291,
Research Areas
Cancer,Cardiology,Cardiovascular,Hedgehog Signaling,Neurobiology,Neurodevelopment,Signal Transduction,Transcription Factor/Regulator,Tumor Suppressors/Oncoproteins
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
Suppressor of fused (SuFu) is a negative regulator of the hedgehog and beta-catenin signaling pathway. It down-regulates GLI1-mediated transactivation of target genes and is part of a corepressor complex that acts on DNA-bound GLI1. SuFu may also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome. Additionally, SuFu sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9UMX1].
UniGene ID
Hs.404089
UniProt ID (eCommerce)
Q9UMX1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.