human SRF shRNA silencing AAV

Name 
AAV-h-SRF-shRNA
Cat No 
shAAV-224323
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human SRF.

shAAV-224323
AAV-h-SRF-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SRF (serum response factor). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
80
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
c-fos serum response element-binding transcription factor; MCM1; minichromosome maintenance 1 homolog; serum response factor; SRF
Description (eCommerce)
Serum response factor (SRF) is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). SRF is required for cardiac differentiation and maturation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P11831].
Description (Vector)
SRF encodes a ubiquitous nuclear protein that stimulates both cell proliferation and differentiation. It is a member of the MADS (MCM1, Agamous, Deficiens, and SRF) box superfamily of transcription factors. This protein binds to the serum response element (SRE) in the promoter region of target genes. This protein regulates the activity of many immediate-early genes, for example c-fos, and thereby participates in cell cycle regulation, apoptosis, cell growth, and cell differentiation. SRF is the downstream target of many pathways; for example, the mitogen-activated protein kinase pathway (MAPK) that acts through the ternary complex factors (TCFs).
Gene ID
6722
Gene Name (eCommerce)
serum response factor
Gene Name (Vector)
serum response factor
Gene Symbol
SRF
HGNC ID
HGNC:11291
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1527
ORF Size (bp)
1527 bp
Protein Name (eCommerce)
Serum response factor
RefSeq ID
NM_003131
RefSeq Synonyms
NM_003131, NM_001292001, BC052572, BC048211,
Research Areas
Angiogenesis,Cancer,Cardiac Development,Cardiology,Cardiovascular,Host-Virus Interactions,Hypoxia,IGF-1 Signaling,Immunology,Insulin Signaling,MAPK Signaling,Neurobiology,Neurodevelopment,PDGF Signaling,Rho Signaling,RNA Binding,Signal Transduction,Transcription Factor/Regulator,Viral Carcinogenesis
Research Areas (Faceted)
cancer,cardiovascular,cell_biology,developmental_biology,immunology,infectious_disease,neurobiology,signal_transduction,transcription_translation
Species
human
Target Sentence
Serum response factor (SRF) is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). SRF is required for cardiac differentiation and maturation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P11831].
UniGene ID
Hs.520140
UniProt ID (eCommerce)
P11831

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.