human SHMT1 shRNA silencing AAV

Name 
AAV-h-SHMT1-shRNA
Cat No 
shAAV-222952
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human SHMT1.

shAAV-222952
AAV-h-SHMT1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SHMT1 (serine hydroxymethyltransferase 1). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
CSHMT; cytoplasmic serine hydroxymethyltransferase; glycine hydroxymethyltransferase; serine hydroxymethyltransferase 1 (soluble); serine hydroxymethyltransferase, cytosolic; serine methylase; SHMT
Description (eCommerce)
Serine hydroxymethyltransferase 1 (SHMT1) is the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm [taken from NCBI Entrez Gene (Gene ID: 6470)].
Description (Vector)
SHMT1 encodes the cellular form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm. SHMT1 is located within the Smith-Magenis syndrome region on chromosome 17. Alternative splicing of this gene results in 2 transcript variants encoding 2 different isoforms. Additional transcript variants have been described, but their biological validity has not been determined.
Gene ID
6470
Gene Name (eCommerce)
serine hydroxymethyltransferase 1
Gene Name (Vector)
serine hydroxymethyltransferase 1
Gene Symbol
SHMT1
HGNC ID
HGNC:10850
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1452
ORF Size (bp)
1452 bp
Protein Name (eCommerce)
Serine hydroxymethyltransferase, cytosolic
RefSeq ID
NM_004169
RefSeq Synonyms
NM_148918, NM_004169, NM_001281786, BC038598, BC022874, BC007979,
Research Areas
Cancer,Metabolism/Metabolic Process,Mitochondrion
Research Areas (Faceted)
cancer,metabolism,cell_biology
Species
human
Target Sentence
Serine hydroxymethyltransferase 1 (SHMT1) is the cytosolic form of serine hydroxymethyltransferase, a pyridoxal phosphate-containing enzyme that catalyzes the reversible conversion of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction provides one-carbon units for synthesis of methionine, thymidylate, and purines in the cytoplasm [taken from NCBI Entrez Gene (Gene ID: 6470)].
UniGene ID
Hs.636044
UniProt ID (eCommerce)
P34896

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.