human SESN2 shRNA silencing AAV

Name 
AAV-h-SESN2-shRNA
Cat No 
shAAV-222726
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human SESN2.

shAAV-222726
AAV-h-SESN2-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SESN2 (sestrin 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
HI95; hypoxia induced gene 95; hypoxia-induced; Hypoxia-induced gene; SES2; SEST2; sestrin-2
Description (eCommerce)
SESN2 functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway. This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. SESN2 may negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1, and may positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1. May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress, and has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein.  [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P58004].
Description (Vector)
SESN2 encodes a member of the sestrin family of PA26-related proteins. The encoded protein may function in the regulation of cell growth and survival. This protein may be involved in cellular response to different stress conditions.
Gene ID
83667
Gene Name (eCommerce)
sestrin 2
Gene Name (Vector)
sestrin 2
Gene Symbol
SESN2
HGNC ID
HGNC:20746
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1443
ORF Size (bp)
1443 bp
Protein Name (eCommerce)
Sestrin-2
RefSeq ID
NM_031459
RefSeq Synonyms
NM_031459, BC033719, BC013304,
Research Areas
Cell Cycle,p53 Signaling,Signal Transduction
Research Areas (Faceted)
cell_biology,cell_cycle,genetics,signal_transduction
Species
human
Target Sentence
SESN2 functions as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway through the GATOR complex. In absence of leucine, binds the GATOR subcomplex GATOR2 and prevents TORC1 signaling. Binding of leucine to SESN2 disrupts its interaction with GATOR2 thereby activating the TORC1 signaling pathway. This stress-inducible metabolic regulator also plays a role in protection against oxidative and genotoxic stresses. SESN2 may negatively regulate protein translation in response to endoplasmic reticulum stress, via TORC1, and may positively regulate the transcription by NFE2L2 of genes involved in the response to oxidative stress by facilitating the SQSTM1-mediated autophagic degradation of KEAP1. May also mediate TP53 inhibition of TORC1 signaling upon genotoxic stress, and has an alkylhydroperoxide reductase activity born by the N-terminal domain of the protein.  [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P58004].
UniGene ID
Hs.469543
UniProt ID (eCommerce)
P58004

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.