human SCNN1A shRNA silencing AAV

Name
AAV-h-SCNN1A-shRNA

Cat No
shAAV-222377

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human SCNN1A.

shAAV-222377

AAV-h-SCNN1A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human SCNN1A (sodium channel epithelial 1 alpha subunit). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

BESC2; ENaCa; ENaCalpha; SCNEA; SCNN1

Description (Vector)

Nonvoltage-gated, amiloride-sensitive, sodium channels control fluid and electrolyte transport across epithelia in many organs. These channels are heteromeric complexes consisting of 3 subunits: alpha, beta, and gamma. This gene encodes the alpha subunit, and mutations in this gene have been associated with pseudohypoaldosteronism type 1 (PHA1), a rare salt wasting disease resulting from target organ unresponsiveness to mineralocorticoids. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.

Gene ID

6337

Gene Name (Vector)

sodium channel epithelial 1 alpha subunit

Gene Symbol

SCNN1A

HGNC ID

HGNC:10599

ORF Size (aa)

2010

ORF Size (bp)

2010 bp

RefSeq ID

NM_001038

RefSeq Synonyms

NM_001159576, NM_001159575, NM_001038, BC062613,

Species

human

UniGene ID

Hs.591047

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.