human RNF4 shRNA silencing AAV

Name 
AAV-h-RNF4-shRNA
Cat No 
shAAV-221430
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human RNF4.

shAAV-221430
AAV-h-RNF4-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RNF4 (ring finger protein 4). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
90
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
RES4-26; SLX5; SNURF
Description (Vector)
RNF4 contains a RING finger motif and acts as a transcription regulator. This protein has been shown to interact with, and inhibit the activity of, TRPS1, a transcription suppressor of GATA-mediated transcription. Transcription repressor ZNF278/PATZ is found to interact with this protein, and thus reduce the enhancement of androgen receptor-dependent transcription mediated by this protein. Studies of the mouse and rat counterparts suggested a role of this protein in spermatogenesis. A pseudogene of this gene is found on chromosome 1.
Gene ID
6047
Gene Name (Vector)
ring finger protein 4
Gene Symbol
RNF4
HGNC ID
HGNC:10067
ORF Size (aa)
573
RefSeq ID
NM_002938
RefSeq Synonyms
NM_002938, NM_001185010, NM_001185009, BC031935,
Species
human
UniGene ID
Hs.66394

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.