human RICTOR shRNA silencing AAV

Name 
AAV-h-RICTOR-shRNA
Cat No 
shAAV-221226
Availability
4-5 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human RICTOR.

shAAV-221226
AAV-h-RICTOR-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RICTOR (RPTOR independent companion of MTOR complex 2). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
AVO3; AVO3 homolog; hAVO3; PIA; pianissimo; rapamycin-insensitive companion of mTOR; TORC2-specific protein AVO3
Description (eCommerce)
Rictor is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6R327].
Description (Vector)
RICTOR and MTOR (FRAP1; MIM 601231) are components of a protein complex that integrates nutrient- and growth factor-derived signals to regulate cell growth (Sarbassov et al., 2004 (PubMed 15268862)).(supplied by OMIM, Mar 2008)
Gene ID
253260
Gene Name (eCommerce)
RPTOR independent companion of MTOR complex 2
Gene Name (Vector)
RPTOR independent companion of MTOR complex 2
Gene Symbol
RICTOR
HGNC ID
HGNC:28611
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
5199
ORF Size (bp)
5199 bp
Protein Name (eCommerce)
Rapamycin-insensitive companion of mTOR
RefSeq ID
NM_152756
RefSeq Synonyms
NM_152756, NM_001285440, NM_001285439, BC144509, BC137164, BC137163, BC029608,
Research Areas
B-cell Receptor Signaling,Cancer,Cytoskeleton,EGFR Signaling,EGFR Signaling in Cancer,Fc Receptor Signaling,FGF Signaling,Immunology,Innate Immunity,mTOR Signaling,Neurobiology,NGF Signaling,PDGF Signaling,PI3K/Akt Signaling,PI3K-AKT Signaling in Cancer,Prostate Cancer,SCF-KIT Signaling,Signal Transduction,Translational Control
Research Areas (Faceted)
cancer,cell_biology,immunology,neurobiology,signal_transduction,stem_cell_biology,transcription_translation
Species
human
Target Sentence
Rictor is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q6R327].
UniGene ID
Hs.407926
UniProt ID (eCommerce)
Q6R327

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.