human RGNEF shRNA silencing AAV

Name 
AAV-h-RGNEF-shRNA
Cat No 
shAAV-221099
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human ARHGEF28.

shAAV-221099
AAV-h-RGNEF-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human ARHGEF28 (Rho guanine nucleotide exchange factor 28). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
shRNA Knockdown (%)
70
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
190 kDa guanine nucleotide exchange factor; p190RHOGEF; p190-RhoGEF; RGNEF; Rho guanine nucleotide exchange factor; Rho guanine nucleotide exchange factor (GEF) 28; rho guanine nucleotide exchange factor 28; Rho interacting protein 2; rho-guanine nucleotide exchange factor; RIP2
Description (eCommerce)
Rho guanine nucleotide exchange factor 28 (p190RhoGEF) Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. p190RhoGEF functions in axonal branching, synapse formation and dendritic morphogenesis. p190RhoGEF also functions in focal adhesion formation, cell motility and B-lymphocytes activation. It may regulate NEFL expression and aggregation and play a role in apoptosis (by similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9HAU5].
Description (Vector)
ARHGEF28 encodes a member of the Rho guanine nucleotide exchange factor family. The encoded protein interacts with low molecular weight neurofilament mRNA and may be involved in the formation of amyotrophic lateral sclerosis neurofilament aggregates. Alternate splicing results in multiple transcript variants.
Gene ID
64283
Gene Name (eCommerce)
Rho guanine nucleotide exchange factor 28
Gene Name (Vector)
Rho guanine nucleotide exchange factor 28
Gene Symbol
ARHGEF28
HGNC ID
HGNC:30322
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
5118
ORF Size (bp)
5118 bp
Protein Name (eCommerce)
Rho guanine nucleotide exchange factor 28
RefSeq ID
NM_001080479
RefSeq Synonyms
NM_001244364, NM_001177693, NM_001080479, BC171850, BC167854, BC157846,
Research Areas
Cytoskeleton,Rho Signaling,RNA Binding,Signal Transduction,Zinc-finger
Research Areas (Faceted)
cell_biology,signal_transduction
Species
human
Target Sentence
Rho guanine nucleotide exchange factor 28 (p190RhoGEF) Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. p190RhoGEF functions in axonal branching, synapse formation and dendritic morphogenesis. p190RhoGEF also functions in focal adhesion formation, cell motility and B-lymphocytes activation. It may regulate NEFL expression and aggregation and play a role in apoptosis (by similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q9HAU5].
UniGene ID
Hs.482521
UniProt ID (eCommerce)
Q8N1W1

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.