human RDH8 shRNA silencing AAV

Name
AAV-h-RDH8-shRNA

Cat No
shAAV-220969

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human RDH8.

shAAV-220969

AAV-h-RDH8-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RDH8 (retinol dehydrogenase 8 (all-trans)). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

PRRDH; SDR28C2

Description (Vector)

All-trans-retinol dehydrogenase (RDH8) is a visual cycle enzyme that reduces all-trans-retinal to all-trans-retinol in the presence of NADPH (Rattner et al., 2000 (PubMed 10753906)). It is a member of the short chain dehydrogenase/reductase family and is located in the outer segments of photoreceptors; hence it is also known as photoreceptor retinol dehydrogenase. It is important in the visual cycle by beginning the rhodopsin regeneration pathway by reducing all-trans-retinal, the product of bleached and hydrolysed rhodopsin (Rando, 2001 (PubMed 11710234)). This is a rate-limiting step in the visual cycle (Saari et al., 1998 (PubMed 9667000)).(supplied by OMIM, Mar 2008)

Gene ID

50700

Gene Name (Vector)

retinol dehydrogenase 8 (all-trans)

Gene Symbol

RDH8

HGNC ID

HGNC:14423

ORF Size (aa)

936

ORF Size (bp)

936 bp

RefSeq ID

NM_015725

RefSeq Synonyms

NM_015725, BC146479, BC140328,

Species

human

UniGene ID

Hs.675522

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.