human RASGRP4 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human RASGRP4.
shAAV-220782
AAV-h-RASGRP4-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human RASGRP4 (RAS guanyl releasing protein 4). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Description (Vector)
RASGRP4 is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene ID
115727
Gene Name (Vector)
RAS guanyl releasing protein 4
Gene Symbol
RASGRP4
HGNC ID
HGNC:18958
ORF Size (aa)
1980
ORF Size (bp)
1980 bp
RefSeq ID
NM_170604
RefSeq Synonyms
NM_170604, NM_001146207, NM_001146206, NM_001146205, NM_001146204, NM_001146203, NM_001146202, BC150202, BC146669,
Species
human
UniGene ID
Hs.130434
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About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.