human RAD9A shRNA silencing AAV

Name 
AAV-h-RAD9A-shRNA
Cat No 
shAAV-220655
Availability
6-8 weeks
Categories
AAV, shRNA-Silencing

This is an AAV expressing shRNA for silencing of Human RAD9A.

shAAV-220655
AAV-h-RAD9A-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RAD9A (RAD9 checkpoint clamp component A). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype
Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids
AAV ITR
AAV2
Promoter
U6 ()
Reporter
eGFP (default), optional CFP, YFP, RFP, mCherry
Storage Buffer
PBS/5% Glycerol
Viral Backbone
Recombinant AAV

Gene Reference Data

Alternate Names
cell cycle checkpoint control protein RAD9A; DNA repair exonuclease rad9 homolog A; hRAD9; RAD9; RAD9 homolog A
Description (eCommerce)
Rad9 is a cell-cycle checkpoint protein with 3’-5’ exonuclease activity and is part of the 9-1-1 complex. The 9-1-1 complex, also called the checkpoint clamp, is a trimeric complex that includes Rad9, Hus1, and Rad1. This complex is recruited by the RAD17/RFC clamp loader to DNA lesions where it aids in the activation of Chk2 by ATM and ATRIP and the subsequent triggering of the checkpoint signaling cascade. In addition to its role in sensing DNA damage, Rad9 also functions in the base excision repair process of DNA repair.
Description (Vector)
RAD9A product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Gene ID
5883
Gene Name (eCommerce)
RAD9 checkpoint clamp component A
Gene Name (Vector)
RAD9 checkpoint clamp component A
Gene Symbol
RAD9A
HGNC ID
HGNC:9827
NCBI Taxonomy ID (eCommerce)
9606.0
ORF Size (aa)
1176
ORF Size (bp)
1176 bp
Protein Name (eCommerce)
Cell cycle checkpoint control protein RAD9A
RefSeq ID
NM_004584
RefSeq Synonyms
NM_004584, NM_001243224, BC014848,
Research Areas
Apoptosis,Cancer,Cell Cycle,DNA Damage/Repair,DNA Replication,Fanconi anemia,Histone Modification,Homologous Recombination,Replication,Signal Transduction
Research Areas (Faceted)
cancer,signal_transduction,cell_biology,cell_cycle,genetics,transcription_translation
Species
human
Target Sentence
Rad9 is a cell-cycle checkpoint protein with 3’-5’ exonuclease activity and is part of the 9-1-1 complex. The 9-1-1 complex, also called the checkpoint clamp, is a trimeric complex that includes Rad9, Hus1, and Rad1. This complex is recruited by the RAD17/RFC clamp loader to DNA lesions where it aids in the activation of Chk2 by ATM and ATRIP and the subsequent triggering of the checkpoint signaling cascade. In addition to its role in sensing DNA damage, Rad9 also functions in the base excision repair process of DNA repair.
UniGene ID
Hs.655354
UniProt ID (eCommerce)
Q99638

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.