human RAD51L1 shRNA silencing AAV

Name
AAV-h-RAD51L1-shRNA

Cat No
shAAV-220647

Availability
6-8 weeks

This is an AAV expressing shRNA for silencing of Human RAD51B.

shAAV-220647

AAV-h-RAD51L1-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RAD51B (RAD51 paralog B). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

R51H2; RAD51L1; REC2

Description (Vector)

RAD51B is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. Rearrangements between this locus and high mobility group AT-hook 2 (HMGA2, GeneID 8091) have been observed in uterine leiomyomata.

Gene ID

5890

Gene Name (Vector)

RAD51 paralog B

Gene Symbol

RAD51B

HGNC ID

HGNC:9822

ORF Size (aa)

1155

ORF Size (bp)

1155 bp

RefSeq ID

NM_002877

RefSeq Synonyms

NM_133510, NM_133509, NM_002877, NM_001321821, NM_001321819, NM_001321818, NM_001321817, NM_001321815, NM_001321814, NM_001321812, NM_001321810, NM_001321809, BC030219,

Species

human

UniGene ID

Hs.172587

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.