human RAD18 shRNA silencing AAV

Name
AAV-h-RAD18-shRNA

Cat No
shAAV-220635

Availability
4-5 weeks

This is an AAV expressing shRNA for silencing of Human RAD18.

shAAV-220635

AAV-h-RAD18-shRNA

Ready-to-use AAV expressing shRNA for silencing of Human RAD18 (RAD18, E3 ubiquitin protein ligase). Available with optional GFP reporter or cell-specific promoter.

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Product Details

AAV Serotype

Available in AAV1, AAV2, AAV3, AAV5, AAV6, AAV8, AAV9, AAV-DJ, AAV-DJ8, AAV-DJ9 and other wildtype/synthetic AAV capsids

AAV ITR

AAV2

Promoter

U6 ()

Reporter

eGFP (default), optional CFP, YFP, RFP, mCherry

shRNA Knockdown (%)

70

Storage Buffer

PBS/5% Glycerol

Viral Backbone

Recombinant AAV

Gene Reference Data

Alternate Names

E3 ubiquitin-protein ligase RAD18; hHR18; hRAD18; postreplication repair protein hRAD18p; postreplication repair protein RAD18; RAD18 homolog; RAD18, S. cerevisiae, homolog; RING finger protein 73; RING-type E3 ubiquitin transferase RAD18; RNF73

Description (eCommerce)

Rad18 is an E3 ubiquitin-protein ligase that associates with the E2 ubiquitin conjugating enzyme UBE2B (Rad6b) and monoubiquitinates PCNA on stalled replication forks. This activity promotes the recruitment of DNA repair polymerases such as DNA polymerase eta (Poleta) and translesion synthesis at DNA lesions.

Description (Vector)

RAD18 is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.

Gene ID

56852

Gene Name (eCommerce)

RAD18, E3 ubiquitin protein ligase

Gene Name (Vector)

RAD18, E3 ubiquitin protein ligase

Gene Symbol

RAD18

HGNC ID

HGNC:18278

NCBI Taxonomy ID (eCommerce)

9606.0

ORF Size (aa)

1488

ORF Size (bp)

1488 bp

Protein Name (eCommerce)

E3 ubiquitin-protein ligase RAD18

RefSeq ID

NM_020165

RefSeq Synonyms

NM_020165, BC001302,

Research Areas

Cell Cycle,DNA Damage/Repair,Replication,Spermatogenesis,Ubiquitination,Zinc-finger

Research Areas (Faceted)

cell_biology,cell_cycle,developmental_biology,genetics,transcription_translation

Species

human

Target Sentence

Rad18 is an E3 ubiquitin-protein ligase that associates with the E2 ubiquitin conjugating enzyme UBE2B (Rad6b) and monoubiquitinates PCNA on stalled replication forks. This activity promotes the recruitment of DNA repair polymerases such as DNA polymerase eta (Poleta) and translesion synthesis at DNA lesions.

UniGene ID

Hs.375684

UniProt ID (eCommerce)

Q9NS91

About Storage Conditions

All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity. We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.