human PUS10 shRNA silencing AAV
This is an AAV expressing shRNA for silencing of Human PUS10.
shAAV-220403
AAV-h-PUS10-shRNA
Ready-to-use AAV expressing shRNA for silencing of Human PUS10 (pseudouridylate synthase 10). Available with optional GFP reporter or cell-specific promoter.
Gene Reference Data
Alternate Names
CCDC139; DOBI
Description (Vector)
Pseudouridination, the isomerization of uridine to pseudouridine, is the most common posttranscriptional nucleotide modification found in RNA and is essential for biologic functions such as spliceosome biogenesis. Pseudouridylate synthases, such as PUS10, catalyze pseudouridination of structural RNAs, including transfer, ribosomal, and splicing RNAs. These enzymes also act as RNA chaperones, facilitating the correct folding and assembly of tRNAs (McCleverty et al., 2007 (PubMed 17900615)).(supplied by OMIM, May 2009)
Gene ID
150962
Gene Name (Vector)
pseudouridylate synthase 10
Gene Symbol
PUS10
HGNC ID
HGNC:26505
ORF Size (aa)
1590
ORF Size (bp)
1590 bp
RefSeq ID
NM_144709
RefSeq Synonyms
NM_144709, NM_001322127, NM_001322124, NM_001322123, BC101706, BC101680,
Species
human
UniGene ID
Hs.368348
About Storage Conditions
All our viral products should be kept at -80°C. At this temperature, the virus will remain stable for 6-12 months (and in some cases, up to 2 years). Once thawed, the product can be stored at 4°C for 2-3 weeks without significant loss of biological activity.
We recommend aliquoting your vectors into low protein binding tubes upon receipt. This helps avoid repeated freeze-thaw cycles, as well as prevent loss of virus. To maintain accurate titer, aliquot in at least 20ul per tube.